Identification and validation of extracellular matrix-related genes in the progression of gastric cancer with intestinal metaplasia

Figure 8 Secreted protein acidic and rich in cysteine expression in tissues and cells. A: The immunohistochemistry results of secreted protein acidic and rich in cysteine (SPARC) in normal gastric mucosa, intestinal metaplasia (IM), and gastric cancer tissue; B: The western blot results of IM markers KLF4, CDX2, and SPARC in GES-1 cell line and chenodeoxycholic acid-induced GES-1 IM cell model; C: The validation results of SPARC knockdown by small interfering (si)-RNA through WB and quantitative real-time polymerase chain reaction; D: The line graph assessing cell proliferation by cell counting kit 8 assay after SPARC knockdown with small interfering (si)-RNA; E: The results of the colony formation assay after SPARC knockdown with si-RNA; F: The results of the cell wound healing assay after SPARC knockdown with si-RNA. ^aP < 0.001, ^bP < 0.01, ^cP < 0.05. NC: Normal control; IM: Intestinal metaplasia; GC: Gastric cancer; IHC: Immunohistochemistry; DMSO: Dimethyl sulfoxide; CDCA: Chenodeoxycholic acid; OD: Optical density; si-NC: Small interfering-normal control; si-SPARC: Small interfering-secreted protein acidic and rich in cysteine; SPARC: Secreted protein acidic and rich in cysteine.