Role and mechanism of sarcosine dehydrogenase in the progression of gallbladder cancer through chemokine pathways

Figure 4 Interaction between sarcosine dehydrogenase and C-X-C motif chemokine ligand 1 in gallbladder cancer and its impact on signaling pathways. A: Real-time quantitative polymerase chain reaction (RT-PCR) detection of the interaction between sarcosine dehydrogenase (SARDH) and C-X-C motif chemokine ligand 1 (CXCL1); B: RT-PCR detection of CXCL1 expression in gallbladder cancer; C: Western blot analysis showing activation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK)1/ERK2 signaling pathways through the SARDH/CXCL1 axis in BGC-SD cells; D: Silencing SARDH and CXCL1 markedly increased the activation levels of p-Akt and p-ERK in cancer cells. SARDH: Sarcosine dehydrogenase; Akt: Protein kinase B; ERK: Extracellular signal-regulated kinase.