SPDL1 inhibition enhances colorectal cancer progression via epidermal growth factor receptor/extracellular signal-regulated kinase pathways

Figure 2 Small interfering RNA knockdown of SPDL1 promotes proliferation, migration and invasion of colorectal cancer cells. A: Western blot showing the expression of SPDL1 protein in a non-cancerous colonic epithelial cell line and colorectal cancer cell lines; B: Quantitative reverse transcription-polymerase chain reaction showing siRNA knockdown of the SPDL1 gene; C: Cell counting kit-8 assay; D: Transwell assay. Data are presented as the mean ± SD from three independent experiments. ^aP < 0.05. ^bP < 0.01. ^dP < 0.0001. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Con: Control; NC: Negative control; si-SPDL1: Small interfering RNA targeting SPDL1; OD: Optical density.