Nitric oxide synthase 1 inhibits the progression of esophageal cancer through interacting with nitric oxide synthase 1 adaptor protein

Figure 8 Effect of nitric oxide synthase 1 and nitric oxide synthase 1 adaptor protein on the expression of nitric oxide, nitrite, and phosphatidylinositol 3-kinase/protein kinase B pathway-related proteins. Enzyme-linked immunosorbent assay to detect nitric oxide and nitrite in different samples. A: TE-15 cell line; B: OE-19 cell line; C and D: Mouse serum; E and F: Tumor tissues; G: Western Blotting was performed to detect the expression of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway-related proteins E-cadherin, phosphorylated (p)-PI3K/PI3K, p-AKT/AKT. Values are mean ± SD. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. n = 3 experiments. oe-NOS1: Overexpression of nitric oxide synthase 1; NO: Nitric oxide; si-NOS1AP: Silencing of nitric oxide synthase 1 adaptor protein; PI3K: Phosphatidylinositol 3-kinase; AKT: Protein kinase B.