Centromere protein A knockdown inhibits rectal cancer through O6-methylguanine DNA methyltransferase/protein tyrosine phosphatase nonreceptor type 4 axis

Figure 4 O6-methylguanine DNA methyltransferase knockdown abolished the effects of centromere protein A knockdown on rectal cancer cell progression. A and B: O6-methylguanine DNA methyltransferase protein levels in SW837 cells were measured by Western blot analysis; C: SW837 cell proliferation was detected using Cell Counting kit-8 assay; D and E: Transwell assay used to detect SW837 cell invasion; F and G: SW837 cell apoptosis examined using flow cytometry; H and I: E-cadherin, N-cadherin and vimentin levels in SW837 cells were examined via Western blot analysis. Data are presented as mean ± SE. ^aP < 0.05; ^bP < 0.01; CENPA: Centromere protein A; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; DNMT1: DNA methyltransferase 1; MGMT: O6-methylguanine DNA methyltransferase; sh-CENPA: Short hairpin RNAs targeting Centromere protein A; sh-MGMT: Short hairpin RNAs targeting O6-methylguanine DNA methyltransferase.