Centromere protein A knockdown inhibits rectal cancer through O6-methylguanine DNA methyltransferase/protein tyrosine phosphatase nonreceptor type 4 axis

Figure 2 Effects of Centromere protein A overexpression and knockdown on the proliferation, invasion, apoptosis and epithelial-mesenchymal transition of rectal cancer cells. A and B: Centromere protein A protein expression in SW837 and SW480 cells was measured using Western blot analysis; C and D: SW837 and SW480 cell proliferation were examined using Cell Counting kit-8 assay; E-G: Cell invasion measured by Transwell assay (E) and quantitative analysis in SW837 (F) and SW480 (G) cells; H-K: Cell apoptosis of SW837 (H and I) and SW480 (J and K) cells examined using flow cytometry; L-O: E-cadherin, N-cadherin and vimentin levels in SW837 (L and M) and SW480 (N and O) cells were measured using Western blot analysis. Data are presented as mean ± SE. ^aP < 0.05; ^bP < 0.01; CENPA: Centromere protein A; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MGMT: O6-methylguanine DNA methyltransferase; sh-CENPA: Short hairpin RNAs targeting Centromere protein A; sh-MGMT: Short hairpin RNAs targeting O6-methylguanine DNA methyltransferase; sh-PTPN4: Short hairpin RNAs targeting protein tyrosine phosphatase nonreceptor type 4.