Involvement of bile acids in cholangiocarcinoma progression via the Hippo-yes-associated protein signaling pathway

Figure 2 Effects of glycochenodeoxycholic acid and deoxycholic acid on cholangiocarcinoma cell proliferation. A: Cell Counting Kit-8 was used to detect the viability of HuCCT1 cells in the control, glycochenodeoxycholic acid (GDCA), deoxycholic acid (DCA), GDCA + YI, and DCA + YI groups; B: 5-ethynyl-2’-deoxyuridine staining was used to detect the DNA replication ability of HuCCT1 cells in the control, GDCA, DCA, GDCA + YI, and DCA + YA groups. Statistical analysis was conducted using one-way ANOVA with Tukey’s post hoc test. Significance is indicated as: ^bP < 0.01 vs control; ^dP < 0.01 vs GDCA; ^fP < 0.01 vs DCA. Data are presented as mean ± SD (n = 3 independent experiments). OD: Optical density; DAPI: 4’,6-diamidino-2-phenylindole; EDU: 5-ethynyl-2’-deoxyuridine; GDCA: Glycochenodeoxycholic acid; DCA: Deoxycholic acid.