Transient receptor potential melastatin 6 and transient receptor potential melastatin 6/7 antagonists suppress colon adenocarcinoma HT-29 cells

Figure 5 Secondary sphere formation assay. A: Serial spheroid formation was assessed by repeated passaging of cancer spheroid (SP)-derived cells treated with or without 100 μmol/L Co(III)hexamine, 200 μmol/L 2-aminoethyl diphenylborinate, or 10 μmol/L Mesendogen. Scale bar = 200 μm; B: The western blot analysis; C: Densitometric analysis of Nanog in SP cells treated with or without 100 μmol/L Co(III)hexamine, 200 μmol/L 2-aminoethyl diphenylborinate, or 10 μmol/L Mesendogen. SP: Spheroid; Co(III)hex: Co(III)hexamine; 2-APB: 2-aminoethyl diphenylborinate; ME: Mesendogen. ^aP < 0.05, ^bP < 0.01 vs the control spheroid group (n = 6).