Transient receptor potential melastatin 6 and transient receptor potential melastatin 6/7 antagonists suppress colon adenocarcinoma HT-29 cells

Figure 1 Expression and subcellular localization of transient receptor potential melastatin 6 and transient receptor potential melastatin 7 in parental and spheroid-derived HT-29 cells. A: The western blot analysis of transient receptor potential melastatin (TRPM) 6; B: The western blot analysis of TRPM7; C: TRPM6 in immunoprecipitation of TRPM6 (IP-TRPM6); D: TRPM7 in IP-TRPM6 of parental HT-29 monolayers and spheroid-derived cells; E: The densitometric analysis of TRPM6; F: The densitometric analysis of TRPM7; G: TRPM6 in IP-TRPM6; H: TRPM7 in IP-TRPM6 of parental HT-29 monolayers and spheroid-derived cells. Total: Total cell; Cyto: Cytosol; Mem: Membrane; SP: Spheroid; TRPM: Transient receptor potential melastatin; IP-TRPM6: Immunoprecipitation of transient receptor potential melastatin 6. ^aP < 0.05, ^bP < 0.01, ^cP < 0.01 vs the corresponding parental cells (n = 6).