5’-transfer RNA halve-lysine-CTT as a promising biomarker for early detection of hepatocellular carcinoma

Figure 7 Target prediction and functional enrichment analysis of 5’-transfer RNA halve-lysine-CTT. A: Schematic diagram illustrating the workflow for 5’-transfer RNA halve-lysine-CTT target prediction. Venn diagram depicting the overlap of predicted target genes identified by miRanda, TargetScan, RIsearch, and RNAhybrid; B: Gene Ontology enrichment analysis of the candidate target genes, highlighting the top enriched terms in biological processes, cellular components, and molecular functions; C: Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the candidate target genes, revealing the most relevant pathways; D: Network visualization of Gene Ontology terms enriched in the candidate target gene set. Only terms with P < 0.01, a minimum count of 3, and an enrichment factor > 1.5 are shown, and they are clustered based on semantic similarity. GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes.