Myc-associated zinc finger protein drives colorectal cancer metastasis through activating ubiquitin like with ring finger protein one

Figure 5 Ubiquitin-like with PHD and RING finger domains 1 regulates colorectal cancer migration, invasion, and epithelial-mesenchymal transition by DNA methylation-mediated suppression of esophageal cancer-related gene 4. A and B: Western blot and quantitative reverse transcription polymerase chain reaction showed esophageal cancer-related gene 4 (ECRG4) level in colorectal cancer (CRC) cells after ubiquitin-like with PHD and RING finger domains 1 (UHRF1) knockdown or combined UHRF1 and ECRG4 knockdown; C and D: Transwell assay images and quantification showed the effects of UHRF1 knockdown and simultaneous knockdown of UHRF1 and ECRG4 on CRC cell migration; E: Western blot of E-cadherin, N-cadherin, vimentin, and snail in CRC cells under UHRF1 knockdown or combined UHRF1 and ECRG4 knockdown. Data are presented as the mean ± SD. The dashes represent the comparisons between groups. The experiments were repeated at least three times independently. ECRG4: Esophageal cancer-related gene 4; UHRF1: Ubiquitin-like with PHD and RING finger domains 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh: Short hairpin; NC: Negative control. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.0001.