Myc-associated zinc finger protein drives colorectal cancer metastasis through activating ubiquitin like with ring finger protein one

Figure 4 Myc-associated zinc finger protein promotes colorectal cancer migration, invasion, and epithelial-mesenchymal transition through ubiquitin-like with PHD and RING finger domains 1 activation. A and B: Transwell assay images and quantification of colorectal cancer cell migration in different groups: Short hairpin (sh)-negative control, sh-Myc-associated zinc finger protein (MAZ), sh-MAZ + overexpression-negative control, and sh-MAZ + overexpression-ubiquitin-like with PHD and RING finger domains 1; C: Western blot showed that protein expression of epithelial-mesenchymal transition markers (E-cadherin, N-cadherin, vimentin, and snail) in colorectal cancer cells. Data are presented as the mean ± SD. The dashes represent the comparisons between groups. The experiments were repeated at least three times independently. MAZ: Myc-associated zinc finger protein; UHRF1: Ubiquitin-like with PHD and RING finger domains 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh: Short hairpin; NC: Negative control; oe: Overexpression. ^bP < 0.01, and ^cP < 0.0001.