Myc-associated zinc finger protein drives colorectal cancer metastasis through activating ubiquitin like with ring finger protein one

Figure 2 Myc-associated zinc finger protein promotes colorectal cancer metastasis in vivo. A: Representative images of lung tissues from sh-negative control and sh-Myc-associated zinc finger protein (MAZ) groups showed the metastatic tumor burden (n = 6); B: Hematoxylin and eosin-stained lung tissue sections showed metastatic tumor nodules, with a quantification bar graph comparing the number of nodules in the sh-negative control and sh-MAZ groups, scale bar: 50 μm; C: Quantitative reverse transcription polymerase chain reaction analysis of MAZ mRNA levels in metastatic tumor nodules; D: Western blot of epithelial-mesenchymal transition markers (E-cadherin, N-cadherin, vimentin, and snail) in metastatic tumor nodules. Data are presented as mean ± SD. The dashes represent the comparisons between groups. The experiments were repeated at least three times independently. MAZ: Myc-associated zinc finger protein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh: Short hairpin; NC: Negative control. ^bP < 0.01, and ^cP < 0.0001.