Effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues

Table 3 Comparison of microbial group alpha diversity using multi- and single-region 16S rRNA gene sequencing in gastric cancer tissues

Group	n	Shannon		Simpson		Chao1
		Paraffinized tissue	Fresh tissue	Paraffinized tissue	Fresh tissue	Paraffinized tissue	Fresh tissue
Multi-region sequencing	59	1.18 ± 0.12	0.68 ± 0.18	0.63 ± 0.08	0.37 ± 0.07	14.67 ± 3.21	3.33 ± 3.21
Single-region sequencing	59	3.08 ± 0.39	2.88 ± 0.40	0.89 ± 0.15	0.88 ± 0.24	199.67 ± 22.50	115.83 ± 31.95
t value	-	35.672	38.917	12.621	15.804	63.194	25.071
P value	-	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001