Helicobacter pylori-induced miR-136 is a potential predictor of early-stage gastric cancer

Figure 5 miR-136 promoted tumorigenesis of Helicobacter pylori-positive gastric cancer cells by targeting PDCD11 in vitro. A and B: MiR-136 mimic significantly attenuated luciferase activity of the wild-type reporter vector. Rather, miR-136 mimic had no effect on the Mut reporter vector via the luciferase assay; C and D: MiR-136 inhibitor significantly increased the luciferase activity of the wild-type reporter vector. Rather, miR-136 inhibitor had no effect on the Mut reporter vector via the luciferase assay; E-I: The expression of PDCD11 mRNA and protein was reduced in SGC-7901 and BGC-823 cells transfected with miR-136 mimic via reverse transcription quantitative PCR and western blot assay. When SGC-7901 and BGC-823 cells were transfected with miR-136 inhibitor, the opposite occurred; J: PDCD11 plasmid cannot be inhibited by miR-136; K-P: The proliferation and migration of gastric cancer cells induced by miR-136 were abrogated by transfecting PDCD11 plasmid. ^aP < 0.01. wt: Wild-type reporter vector; mut: Mutant reporter vector.