Helicobacter pylori-induced miR-136 is a potential predictor of early-stage gastric cancer

Table 2 Sequences of primers and microRNAs

Gene		Primer sequence (5’-3’)
miR-136	Forward	ACACTCCAGCTGGGACTCCATTTGTTTTG
	Reverse	CTCAACTGGTGTCGTGGAGTCGGCAATCAGTTGAGTCCATCAT
U6	Forward	CTCGCTTCGGCAGCACA
	Reverse	AACGCTTCACGAATTTGCGT
PDCD11	Forward	GAAGCTACCATGGTTGCAACAGTACAGG
	Reverse	CCTGTACTGTGAATTCGATGGTAGCTTCC
GAPDH	Forward	GAGGGATTTTTGTTTTTATGTTTTG
	Reverse	TATATACACCAACAACTCTCTCCAA
miR-136 mimics	Forward	ACUCCAUUUGUUUUGAUGAUGGA
	Reverse	UCCAUCAUCAAAACAAAUGGAGU
miR-NC	Forward	UUUGUACUACACAAAAGUACUG
	Reverse	CAGUACUUUUGUGUAGUACAAA
miR-136 inhibitor		UCCAUCAUCAAAACAAAUGGAGU
NF-κB WT		GGGGCAACGGCAGGGGAATTCCCCTCTCCTTA
NF-κB Mut		GGGGCAACGGCAGATCTATTCCCCTCTCCTTA

NF-κB: Nuclear factor kappa-B; WT: Wild-type; Mut: Mutated binding site; NC: Control.