METTL5 promotes gastric cancer progression via sphingomyelin metabolism

Figure 6 Supplementing s1p could partially rescue the phenotype caused by METTL5 knockdown. A: Enzyme-linked immunosorbent assay to detect the content of sphingosine in HGC-27, AGS cells after METTL5 knockdown and METTL5 overexpression; B: Cell Counting Kit-8 assay to detect cell proliferation ability after addition of s1p in HGC-27 and AGS cells with METTL5 knockdown; C: Clone formation assay to detect cell clone formation ability after the addition of s1p in HGC-27 and AGS cells with METTL5 knockdown; D: The wound-healing motility assay to detect migration formation ability after the addition of s1p in HGC-27 and AGS cells with METTL5 knockdown; E: Transwell assay to detect invasion formation ability after the addition of s1p in HGC-27 and AGS cells with METTL5 knockdown. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.