Two missense STK11 gene variations impaired LKB1/adenosine monophosphate-activated protein kinase signaling in Peutz-Jeghers syndrome

Figure 2 Multiple software predict the pathogenicity of these variations. A-D: Three-dimensional model of the protein structure of liver kinase B1 and amino acid molecular structure formula. These variations result in significant changes in the properties of amino acids, which may be harmful; E: Evolutionary conservation shows that the variation sites (p.R297G and p.L245F) are highly conserved across different species; F: Four software assessed the pathogenicity of these variations, all of which were likely pathogenic or pathogenic. “R” represents arginine; “G” represents glycine; “L” represents leucine; and “F” represents phenylalanine.