MicroRNA-298 determines the radio-resistance of colorectal cancer cells by directly targeting human dual-specificity tyrosine(Y)-regulated kinase 1A

Figure 4 Human dual-specificity tyrosine(Y)-regulated kinase 1A is microRNA-298’s target. A: A schematic diagram depicts the putative binding loci of microRNA-298 (miR-298) within the 3’ untranslated region (3’UTR) of the human dual-specificity tyrosine(Y)-regulated kinase 1A (DYRK1A) gene; B: A luciferase reporter assay was carried out to assess the direct association of miR-298 with DYRK1A in HT-29. These cells were co-transfected with the miR-298 mimic plus a luciferase reporter construct containing either the wild-type (WT) DYRK1A 3’UTR or a mutant (Mut) version that disrupted the putative miR-298 binding sites; C: Following negative control (NC), miR-298 inhibitor, or miR-298 mimic transfection, and Western blotting was carried out to determine DYRK1A protein expression in HT-29 cells; D: HT-29 cells were subjected to NC, or miR-298 mimic transfection, or miR-298 mimic plus pcDNA-DYRK1A vector co-transfection, followed by western blotting determination of DYRK1A and p53 binding protein 1 expressions. ^aP < 0.05, ^bP < 0.01. miR-298: MicroRNA-298; IR: Ionizing radiation; DYRK1A: Human dual-specificity tyrosine(Y)-regulated kinase 1A; NC: Negative control.