Verteporfin fluorescence in antineoplastic-treated pancreatic cancer cells found concentrated in mitochondria

Figure 3 Apoptosis of pancreatic cancer cells after incubation with doxorubicin and gemcitabine. A: Apoptotic rates of AsPC-1 cells after incubation with different concentrations of doxorubicin (DOX) (nil, 0.2, 0.4, 0.6, 0.8, 1 μmol/L) for 48 h; B: Apoptotic rate of BxPC-3 cells after incubation with different concentrations of DOX (nil, 0.2, 0.4, 0.6, 0.8, 1 μmol/L) for 48 h; C: AsPC-1 pancreatic cancer (PC) cells were incubated with 0.8 μmol/L DOX for 48 h and observed by confocal laser scanning microscopy after staining with Annexin V-FITC and propidium iodide (PI); D: BxPC-1 PC cells were incubated with 0.4 μmol/L DOX for 48 h and observed by confocal laser scanning microscopy after staining with Annexin V-FITC and PI; E: Apoptotic rate of AsPC-1 cells after incubation with different concentrations of gemcitabine (GEM) (nil, 10, 30, 50, 70 μmol/L) for 72 h; F: Apoptotic rates of BxPC-3 cells after incubation with different concentrations of GEM (nil, 10, 30, 50, 70 μmol/L) for 72 h; G: AsPC-1 cells were incubated with 30 μmol/L of GEM for 72 h and observed by confocal laser scanning microscopy after staining with Annexin V-FITC and PI; H: BxPC-1 cells were incubated with 50 μmol/L of GEM for 72 h and observed by confocal laser scanning microscopy after staining with Annexin V-FITC and PI. Experiments were performed in triplicate.