Prohibitin 1 inhibits cell proliferation and induces apoptosis via the p53-mediated mitochondrial pathway in vitro

Figure 4 Prohibitin 1 regulates cell cycle progression in hepatocellular carcinoma cells. A-H: The p53, p21, Cyclin A2, Cyclin E1, and CDK2 mRNA and protein levels in HepG2 and SMMC-7721 cells transfected with pEGFP-PHB1 or shRNA-PHB1 for 48 h were determined by real-time PCR (A-D) and Western blot (E-H), respectively. The data are presented as the mean ± SD of three independent experiments with similar results. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001 vs control and empty vector/shRNA-control.