Prohibitin 1 inhibits cell proliferation and induces apoptosis via the p53-mediated mitochondrial pathway in vitro

Figure 1 Prohibitin 1 expression in hepatocellular carcinoma patients. A: Serum levels of Prohibitin 1 (PHB1) in hepatocellular carcinoma (HCC) patients (n = 42) and healthy individuals (n = 42) were measured by ELISA; B-D: PHB1 mRNA levels investigated using real-time PCR (B), rate of positive PHB1 expression (C), and immunohistochemical staining for PHB1 measured via immunohistochemistry (D) in normal tissues from healthy individuals (n = 30) as well as in peritumoral tissues and tumour tissues from HCC patients (n = 30, 200 × and 400 ×). The data are presented as the mean ± SEM of three independent experiments with similar results. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs healthy individuals. PT: Peritumoral tissues; TT: Tumour tissues; NT: Normal tissues; HCC: Hepatocellular carcinoma; PHB1: Prohibitin 1.