Cellular senescence throws new insights into patient classification and pharmacological interventions for clinical management of hepatocellular carcinoma

Figure 10  Suppression of TRNP1 induces apoptosis and senescence of hepatocellular carcinoma cells and attenuates tumor growth. A–C: Flow cytometry for measuring the apoptotic rate of in SMMC-7721 and HepG2 cells with transfection of specific siRNAs of TRNP1; D-F: SA-β-galactosidase (SA-β-Gal) staining for evaluating senescence of transfected hepatocellular carcinoma cells. Bar, 10 μm; G–K: Immunoblotting of p16 and p21 expression in transfected cells; L: Representative photographs of tumors from mice of si-NC, si-TRNP1#1 and si-TRNP1#2 groups; M: Calculation of tumor volume in above groups. ^cP < 0.001; ^dP < 0.0001.