RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer

Figure 5 Cleavage and polyadenylation factor 6 regulates integrin binding sialoprotein to affect gastric cancer progression. Cells were divided into the si-NC, si-cleavage and polyadenylation factor 6 (si-CPSF6), and si-CPSF6 + OV-integrin binding sialoprotein (IBSP) groups. A and B: The mRNA and protein expression of CPSF6 and IBSP was detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively; C: Cell viability was examined by Cell Counting Kit-8 assay; D and E: Cell invasion and migration were tested by transwell assay; F: Cell apoptosis was measured by flow cytometry; G: The protein expression levels of NLR family pyrin domain containing 3, cleaved caspase-1, interleukin-18 (IL-18), and IL-1β were detected by Western blot; H-J: Tumor size, volume, and weight were measured. ^bP < 0.01. IBSP: Integrin binding sialoprotein; CPSF6: Cleavage and polyadenylation factor 6; NLRP3: NLR family pyrin domain containing 3; IL: Interleukin.