RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer

Figure 3 The RNA-binding protein cleavage and polyadenylation factor 6 binds to the 3’-untranslated region of integrin binding sialoprotein and regulates its expression. A: Potential RNA-binding proteins that can bind to integrin binding sialoprotein (IBSP) were analyzed through bioinformatic analysis; B: The levels of cleavage and polyadenylation factor 6 (CPSF6) in stomach adenocarcinoma; C: The mRNA expression of CPSF6 was detected in GC tissues and normal adjacent tissues by real-time quantitative polymerase chain reaction (RT-qPCR); D: The correlation between CPSF6 and IBSP was verified; E and F: The binding ability between CPSF6 and IBSP was confirmed by luciferase reporter and RNA immunoprecipitation chip assays; G and H: The mRNA and protein expression of CPSF6 and IBSP was measured in the si-NC, si-CPSF6#1, and si-CPSF6#2 groups by RT-qPCR and Western blot, respectively. ^aP < 0.05, ^bP < 0.01.