RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer

Figure 2 Integrin binding sialoprotein downregulation suppresses cell proliferation, migration, and invasion and facilitates pyroptosis. A and B: The mRNA and protein expression levels of integrin binding sialoprotein (IBSP) were assessed in the si-NC, si-IBSP#1, and si-IBSP#2 groups by real-time quantitative polymerase chain reaction and Western blot in MKN45 and HGC-27 cells, respectively; C: Cell viability was detected after silencing IBSP by Cell-Counting Kit-8 assay in MKN45 and HGC-27 cells; D and E: Cell invasion and migration were evaluated by Transwell assay in MKN45 and HGC-27 cells; F: Pyroptosis was measured after IBSP knockdown by flow cytometry in MKN45 and HGC-27 cells; G: The protein expression levels of NLR family pyrin domain containing 3, cleaved caspase-1, interleukin-18 (IL-18), and IL-1β were examined by Western blot after suppressing IBSP in MKN45 and HGC-27 cells. ^bP < 0.01. IBSP: Integrin binding sialoprotein; IL: Interleukin; NLRP3: NLR family pyrin domain containing 3.