Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

Figure 3 miR-188-3p induction of apoptosis and autophagy of gastric cancer cells. A and B: Flow cytometric/Annexin V-PE/7-AAD assay. AGS and BGC-823 cells were seeded in six-well plates for transfection with miR-188-3p mimics, inhibitor, or their NC and assayed for tumor cell apoptosis. ^bP < 0.01; C: qRT-PCR. AGS and BGC-823 cells were plated for transfection with miR-188-3p mimics, inhibitor, or their NC and the qRT-PCR assay was performed. ^bP < 0.01 and ^cP < 0.001; D and E: Western blot. AGS and BGC-823 cells were plated in 10 cm dishes for transfection with miR-188-3p mimics, inhibitor, or their NC and Western blot was conducted for the detection of Beclin-1 and LC3-II/LC3-I proteins.