Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

Figure 2 miR-188-3p inhibition of gastric cancer cell growth and invasion. A and B: 5-ethynyl-2-deoxyuridine (EdU) incorporation assay. AGS and BGC-823 cells were grown and transfected with miR-188-3p mimics, inhibitor, or their NC for 48 h. The EdU assay was then performed. The red fluorescence indicates EdU-positive cells, while the blue fluorescence indicates cell nuclei of all cells. The proliferation rate of tumor cells after transfection with miR-188-3p mimics was lower than that of tumor cells transfected with m-NC (^cP < 0.001 vs m-NC or i-NC); C and D: Plate cloning assay. AGS and BGC-823 cells were transfected with miR-188-3p mimics, inhibitor, or their NC and then assayed using the plate-cloning assay. ^cP < 0.001.