Potential of damage associated molecular patterns in synergising radiation and the immune response in oesophageal cancer

Figure 2 HMGB1 and calreticulin are expressed at higher levels under 5% Oxygenation in the OE33P cell line, with a higher expression under normal oxygenation in the OE33R cell line. OE33P and OE33R cell lines were screened for the surface expression of HMGB1 and calreticulin by flow cytometry. 21% = Normal oxygenation, 5% = mild hypoxia, 0.5% = severe hypoxia. Bolus dosing was administered once daily over three consecutive days. Staining of cancer cells took place 24 h after last fraction of radiation. Tukey’s multiple comparison testing. Graph shows mean % expression (± SEM) (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001 vs oxygen levels for each radiation dosing regimen; ^eP < 0.05, ^fP < 0.01, ^gP < 0.001, ^hP < 0.0001 vs dosing with 0 Gy at 21% O₂; ⁱP < 0.05, ^jP < 0.01, ^kP < 0.001 vs dosing with 0 Gy at 5% O₂; ^lP < 0.05, ^mP < 0.01, ⁿP < 0.001 vs dosing with 0 Gy at 0.5% O₂.