Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells

Figure 5 Effects of Xiaojianzhong decoction on gastric mucosal hypoxia in N-methyl-N’-nitro-N-nitrosoguanidine-induced gastric precancerous lesions rats. A and B: Western blot analysis was performed to detect hypoxia-inducible factor 1α (HIF-1α), E1B19000 interacting protein 3 (Bnip-3) protein expression (n = 3); C and D: HIF-1α, Bnip-3 mRNA expression was determined using real-time polymerase chain reaction analysis (n = 3); E-G: Hypoxia-induced autophagy-related protein HIF-1α, Bnip-3 expression was determined using immunofluorescence (n = 5); HIF-1α, Bnip-3 proteins positive score was determined using Image J. Data are expressed as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs model group. XJZ-L: Low dose of Xiaojianzhong decoction; XJZ-M: Middle dose of Xiaojianzhong decoction; XJZ-H: High dose of Xiaojianzhong decoction; HIF-1α: Hypoxia-inducible factor 1α; Bnip-3: B cell lymphoma/Leukemia-2 and adenovirus E1B19000 interacting protein 3.