Dopamine and cyclic adenosine monophosphate-regulated phosphoprotein with an apparent Mr of 32000 promotes colorectal cancer growth

Figure 2 Dopamine and cyclic adenosine monophosphate regulated phosphoprotein with an apparent Mr of 32000 promotes colorectal cancer proliferation in vitro. A and B: Detecting the expression level of dopamine and cyclic adenosine monophosphate regulated phosphoprotein with an apparent Mr of 32000 (DARPP-32) by western blot analysis in HCT116/SW480 stable cell lines overexpressing DARPP-32 and in HT-29/LOVO cell lines with DARPP-32 knockdown; C-G: DARPP-32 overexpression promoted the growth of HCT116 and SW480 cells by ethynyl-2′-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) (black scale bars, 100 μm); H-K: Representative images of the proliferative capabilities of cells in HT-29 and LOVO cells with small interfering RNA (siRNA) knockdown of DARPP-32 detected by EdU and CCK-8 (black scale bars, 100 μm). ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Negative control.