Hsa_circ_0001658 accelerates the progression of colorectal cancer through miR-590-5p/METTL3 regulatory axis

Figure 2 Effects of circ_0001658 on the multiplication, apoptosis and cell cycle of colorectal cancer cells. A: Circ_0001658 expressions in normal colorectal epithelial cells and colorectal cancer cell lines (HT29, SW480, LoVo, and DLD-1) were under the determination of quantitative real-time polymerase chain reaction (qRT-PCR); B: Negative control (NC) and circ_0001658 overexpressing plasmids were transfected into HT29 cells respectively, and si-NC, si-circ_1 or si-circ-2 were transfected into SW480 cells. The efficiency was then probed by qRT-PCR; C-E: Cell countering kit-8 assay and flow cytometry ensured the analysis on the effect of overexpression or knockdown of circ_0001658 on the proliferation, apoptosis and cell cycle of HT29 and SW480 cells. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. NC: Negative control; circ-OE: Circ_0001658 plasmid; si-NC: siRNA negative control; OD: Optical density.