Differences of core genes in liver fibrosis and hepatocellular carcinoma: Evidence from integrated bioinformatics and immunohistochemical analysis

Figure 2 Expression of hub genes in the liver tissue of CCl₄-induced mouse mice. A: Masson’s trichrome and HE staining of control and CCl₄-induced liver cirrhosis mouse liver tissues; B: Collagen area in Masson’s trichrome staining (n = 7 or 8); C: The mRNA expression levels of 10 hub genes of control and CCl₄-induced cirrhosis mouse liver tissues (n = 6); D: The protein expression levels of α-SMA, Decorin (DCN), Dermatopontin (DPT), and SRY-box transcription factor 9 (SOX9) in liver tissues of mice in two groups. The right panel showed the result of quantitative analysis (n = 4). All data were presented as mean ± SE. Two-tailed Student’s t test were performed. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001.