RNA-Seq profiling of circular RNAs in human colorectal cancer 5-fluorouracil resistance and potential biomarkers

Figure 5 Comparison of mRNA expression profiles in 5-fluorouracil-resistant colorectal cancer cell lines. A and B: The volcano plot shows the comparison of the mRNA expression profiles between the parental and 5-fluorouracil (5-Fu) resistant HCT116 and Lovo cell lines; C: Heatmap of the clustering analysis indicating differences in mRNA expression profiles in both paired cell lines; D: Analysis of interactions between mRNAs that were upregulated in both paired 5-Fu-resistant cell lines and the potential target genes predicted by the circRNA-miRNA-mRNA regulatory network; E: Relative mRNA expression in the 2 paired cell lines was measured using quantitative real-time polymerase chain reaction assays.