Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer

Figure 6 Silencing signal transducer and activator of transcription 3 decreases transforming growth factor-β1 protein expression in RKO cells. A: Protein levels of phosphorylated-signal transducer and activator of transcription 3 (p-STAT3) in RKO cells transfected with STAT3 siRNA plasmid by western blot assay; B: Knockdown of STAT3 decreased the levels of transforming growth factor-β1 protein but not ELL-associated factor 2 protein in RKO cells. The data are shown as the mean ± SD. ^aP < 0.01. RKO-C: The group of RKO control cells; STAT3-si: The group of RKO cells transfected with signal transducer and activator of transcription 3 siRNA plasmid. STAT3-NC: The group of RKO cells transfected empty vector; p-STAT3: Phosphorylated-signal transducer and activator of transcription 3; TGF-β1: Transforming growth factor-β1; EAF2: ELL-associated factor 2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.