Highly accurate colorectal cancer prediction model based on Raman spectroscopy using patient serum

Figure 1 Schematic of the confocal micro-Raman spectrometer used in this study. A nomadic Raman microscope with an excitation laser at a wavelength of 1064 nm was used in this study. A 20 × magnifying objective lens with a correction collar with near-infrared microscopy (LCPLN20XIR; Olympus Corporation, Tokyo, Japan) and a 2048 × 64 pixel thermoelectric cooled indium gallium arsenide (InGaAs), charge-couple device (CCD) detector, with a spectral range of 100-3200 cm^-1 (grating 4 cm^-1) were used to record the spectra.