LncRNA C9orf139 can regulate the growth of pancreatic cancer by mediating the miR-663a/Sox12 axis

Figure 3 Effect of long non-coding RNA C9orf139 and miR-663a on pancreatic cancer cell behaviors. A: Dual-luciferase reporter assay revealed a target binding between C9orf139 and miR-663a; B: RNA immunoprecipitation assay showed the enrichment of C9orf139 and miR-663a in PaCa-2 cells containing Ago2 antibody, with IgG antibody as a negative control; C: RNA pull-down assay was performed in PaCa-2 cells with biotin-labeled miR-663a, and then qRT-PCR was conducted to measure C9orf139 expression; D: Expression of miR-663a in patients with pancreatic cancer; E: Correlation analysis between miR-663a and C9orf139 in patients with pancreatic cancer; F: Relative expression of miR-663a in cells after the transfection; G: Cell proliferation after the transfection; H: Cell invasion after the transfection; I: The 24 h migration after the transfection; J: Cell apoptosis after the transfection. ^bP < 0.01 compared with the control group; ^eP < 0.001 compared with the control group.