Plasma and wound fluid levels of eight proangiogenic proteins are elevated after colorectal resection

Figure 4 Enzyme-Linked Immuno-Sorbent Assays determined preoperative and postoperative placental growth factor levels of plasma and wound fluid levels in colorectal surgery benign patients and malignant patients. A: Colorectal surgery benign patients. Preoperative vs Postoperative plasma: Preoperative vs Postoperative day 1 (n = 26), ^fP < 0.01; Preoperative vs Postoperative day 3 (n = 23), ^fP < 0.01; Preoperative vs Postoperative day 7-13 (n = 16), ^eP < 0.05; Preoperative vs Postoperative day 14-20 (n = 6), ns. Plasma vs wound fluid: Postoperative day 1 (plasma, n = 26) vs Postoperative day 1 (wound fluid, n = 30), ^fP <0.01; Postoperative day 3 (plasma, n = 23) vs Postoperative day 3 (wound fluid, n = 28), ^fP <0.01; Postoperative day 7-13 (plasma, n = 16) vs Postoperative day 7-13 (wound fluid, n = 14), ^fP < 0.01; POD14-20 (plasma, n = 6) vs POD14-20 (wound fluid, n = 4), ^eP < 0.05. Plasma and wound fluid protein levels are expressed as median and 75% quartile range (Statistical significance is expressed as ^eP < 0.05, ^fP < 0.0.1); B: Colorectal surgery malignant patients. Preoperative vs Postoperative plasma: Preoperative vs Postoperative day 1 (n = 35), ^dP <0.01; Preoperative vs Postoperative day 3 (n = 28), ^dP < 0.01; Preoperative vs Postoperative day 7-13 (n = 17), ^dP < 0.01; Preoperative vs Postoperative day 14-20 (n = 7), ^cP < 0.05. Plasma vs wound fluid: Postoperative day 1 (plasma, n = 35) vs Postoperative day 1 (wound fluid, n = 33), ^dP < 0.01; Postoperative day 3 (plasma, n = 28) vs Postoperative day 3 (wound fluid, n = 29), ^dP < 0.01; Postoperative day 7-13 (plasma, n = 17) vs Postoperative day 7-13 (wound fluid, n = 20), ^dP < 0.01; Postoperative day 14-20 (plasma, n = 7) vs Postoperative day 14-20 (wound fluid, n = 8), ^dP < 0.01. Plasma and wound fluid protein levels are expressed as median and 75% quartile range. Statistical significance is expressed as ^cP < 0.05, ^dP < 0.01). Preop: Preoperative; POD: Postoperative day; PLS: Plasma; WFL: Wound fluid; PLGF: Placental growth factor.