MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation

Figure 5 PBX3 was a direct target of miR-320a. A: Banding region between miR-320a and PBX3 was predicted by microRNA.org; B: Relative luciferase activities of PBX3 in wild type and mutant; C: PBX3 protein level in gastric cancer (GC) cells after transfection with miR-320a mimics, scramble mimics, miR-320a inhibitor, scramble inhibitor or no transfection for 48 h; D: Protein level of PBX3 in GC tissues and matched adjacent normal tissues. Reference protein was set using GAPDH. C: Cancer tissues; N: Normal tissues; ^aP < 0.05; ^bP < 0.01.