Potential of casein kinase I in digestive cancer screening

Figure 4 Pharmacological inhibition of CKIepsilon/δ reduces insulin-induced Akt phosphorylation in PDAC cells. Cells were serum starved overnight and then incubated in 10 μg/mL insulin-containing media for 20 min in the presence or absence of the CKIepsilon/δ inhibitor IC261 (40 μmol/L). Akt activation was measured by phosphorylation at T308 and S473. Bands were quantified using Image J software and values were normalized against total Akt.