Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2

Figure 6 Specific antigen was precipitated from HepG2 lysate by mouse IgG1 (1), or anti-glypican-3 (2), or mAb 1E4-1D9 (3). The antigen was electrophoresed in 10% sodium dodecylsulfate-polyacrylamide gel electrophoresis at 200V for 45 min in non-reduced condition and blotted onto PVDF membrane. The antigen was probed with mouse IgG1, isotype control (A), or anti-glypican-3 (B), or mAb 1E4-1D9 (C), compared to HepG2 lysate probed with anti-glypican-3 (D). The reaction was detected by horseradish peroxidase-conjugated rabbit anti-mouse Igs and visualized by SuperSignal™ West Pico Chemiluminescent Substrate. PVDF: Polyvinyl difluoride.