Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2

Figure 3 Specific antigen was immunoprecipitated from HepG2 cell lysate by mAb 1E4-1D9-immobilized protein G agarose beads. Eluate was separated under non-reducing conditions in 10% sodium dodecylsulfate-polyacrylamide gel electrophoresis at 200 V for 45 min. One gel was blotted onto polyvinyl difluoride membrane and probed with mAb 1E4-1D9. A: The reaction was detected by HRP-conjugated rabbit anti-mouse Igs and signal was developed by SuperSignal™ West Pico Chemiluminescent Substrate; B: Another gel was stained with Coomasie Brilliant Blue and protein band of 135 kD was cut and sent for amino acid analysis (B).