Lack of hepcidin expression attenuates steatosis and causes fibrosis in the liver

Figure 7 Protein expression levels of phosphorylated Jun N-terminal kinase and alpha smooth muscle actin in Hamp1 floxed and knockout mice fed with high fat or regular diets for 3 or 7 mo. The expression levels of alpha smooth muscle actin (αSMA) (A) and phosphorylated Jun N-terminal kinase (p-JNK) (B) proteins in the livers of floxed and knockout mice fed with regular or high fat sucrose (HFS) diets for 3 or 7 mo was determined by western blotting, as described in Material and Methods. An anti-gapdh antibody was used as control to determine equal protein loading; Gapdh: Glyceraldehyde 3-phosphate dehydrogenase.