Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver

Table 1 Composition of the preservation solutions ViaSpan^® and BG35

	ViaSpan®	BG35
Impermeants (mmol/L)
Lactobionate	100
Gluconate		100
Raffinose	30
Buffers (mmol/L)
KH2PO4	25	2.5
BES		50
Substrates (mmol/L)
Allopurinol	1	1
Glutathione	3	3
Adenosine	5	5
Glycine		15
MgSO4	5	5
Colloids (g/L)
HES	50
PEG 35000		40
pH	7.40	7.40
Osm (mOsm/kg water)	320 ± 4	339 ± 4

Dexamethasone 16 mg/L, insulin 40 UI/L and penicillin G 200000 UI/L were added to ViaSpan^® before use. Streptomycin 0.25 mg/mL and penicillin G 10 UI/mL were added to BG35 before use. All the solutions were bubbled with 100 % N2 for 45 min at 0 °C before use. BES: N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid; HES: Hydroxyethyl starch; PEG: Polyethyleneglycol.