Interplay between microRNA-17-5p, insulin-like growth factor-II through binding protein-3 in hepatocellular carcinoma

Figure 3 Impact of microRNA-17-5p on free insulin-like growth factor-II protein in HuH-7 cells. HuH-7 cells were transfected with miR-17-5p mimics or inhibitors. The free IGF-II protein was measured in media of mimicked and antagonized HuH-7 cells using an IGF-II ELISA Kit. Free IGF-II protein, measured at λmax = 450, was found to be significantly increased upon mimicking of miR-17-5p expression compared to mock untransfected cells (P = 0.0339), while inhibitors of miR-17-5p showed no effect on the levels of free IGF-II protein levels compared to mock cells. Unpaired t-test was performed. ^aP < 0.05. IGF-II: Insulin-like growth factor-II; miR-17-5p: MicroRNA-17-5p.