Management of hepatocellular carcinoma: Predictive value of immunohistochemical markers for postoperative survival

Table 2 p53 antibody used in different studies in this review

Ref.	Clone	Source	Dilution	Antigen retrieval	Cut-off value1
Tseng et al[11]	DO-7	DAKO	1:100	Citrate buffer	> 5% nuclear p53 staining
Hu et al[13]	DO-7	DAKO	1:1000	Citrate buffer	> 10% nuclear p53 staining
Kang et al[14]	DO-7	DAKO	1:100	Citrate buffer	> 5% nuclear p53 staining
Stroescu et al[16]	DO-7	DAKO	Not reported	Citrate buffer	< 24% nuclear p53 staining
Sung et al[17]	Bp53-12	Zymed	1:80	Citrate buffer	> 5% nuclear p53 staining
Qin et al[19]	DO-7	DAKO	Not reported	Citrate buffer	≥ 10% nuclear p53 staining
Guo et al[20]	CM1	SDC	1:2000	Citrate buffer	> 5% nuclear p53 staining
Umemura et al[21]	DO-7	DAKO	1:50	Citrate buffer	≥ 10% nuclear p53 staining

¹Immunohistochemical cut-off value indicates the percentage of cells with p53 positively staining nuclei. DAKO: Dako Denmark A/S, Glostrup, Denmark; Zymed: Zymed Lab Inc, CA, United States; SDC: San Diego, CA, United States.