Role of autophagy in differential sensitivity of hepatocarcinoma cells to sorafenib

Figure 2 Autophagic response to sorafenib. A: LC3 expression in hepatocellular carcinoma cells was analyzed in the presence of SFN for up to 6 h. Representative blot (left panel) and quantitative analysis of LC3-II by densitometry (middle and right panel). ^aP < 0.05; B: LC3 expression was analyzed in the presence of SFN with and without CQ to determine autophagy flux after 4 and 24 h; C: Autophagic response to nutrient-rich (control) or nutrient-depleted medium was analyzed by immunoblotting of LC3 in Hep3B and Huh7 cells for up to 4 h; D: LC3 expression was analyzed in the presence of 5 nmol/L RAPA after 4 h treatment with and without CQ to examine autophagy flux. NS: Non-significant; SFN: Sorafenib; CQ: Chloroquine; RAPA: Rapamycin.