Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH

Figure 4 Thin layer chromatography method. Samples were freeze-dried, extracted, centrifuged, and the supernatant was transferred to a new vessel and dried under gaseous nitrogen. In the meantime plates were prewashed and activated. Standard solutions and samples were subjected to the prewashed and activated thin layer chromatography (TLC) plates and developed with the first eluant system to half of the plate. After drying the TLC plate was subjected to a second eluant system in the same direction up to the top of the plate. After drying of the plates, lipids were visualised with 2’,7’-dichlorofluorescein (DCF) and afterwards with sulfuric acid/ethanol and heating to 160 °C.