Identification of cellular genes showing differential expression associated with hepatitis B virus infection

Table 1 Microarray and quantitative reverse transcription-polymerase chain reaction for candidate genes

No.	Genes	Microarray3		qRT-PCR4		Accession number5
		cy3/cy5	cy5/cy3	/β2M	/HPRT1
1	CSTA1	0.35	0.26	0.23	0.23	NM_005213
2	APM21	0.46	0.31	0.22	0.23	NM_006829
3	SLPI1	0.47	0.31	0.08	0.09	NM_003064
4	CTGF1	3.36	2.026	3.49	3.93	NM_001901
5	NADE1	3.89	2.85	3.99	4.48	AF187064
6	TTR1	5.13	3.07	4.6	5.3	NM_000371
7	ARL3	0.59	0.52	0.54	0.62	NM_004311
8	GCNT3	3.2	1.97	1.74	1.95	NM_004751
9	NRF-1	1.42	2.16	0.98	1.12	L22454
10	KIAA180812	0.16	0.85	0.34	0.4	AB058711
11	HSPC1592	0.9	0.04	0.66	0.77	NM_014181
12	MAP2K62	11.08	3.76	1.68	1.89	NM_002758
13	SKAP212	13.45	2.33	2.03	2.34	NM_003930

¹huHpS > 2x huHB4 or 2x huHpS < huHB4, respectively, in both microarray and qRT-PCR;

²flip-inconsistent spots;

³a signal intensity ratio of huHB4/huHpS;

⁴relative expression in huHB4 against in huHpS normalized by beta 2-microglobin (β2M) or hypoxanthine phosphoribosyltransferase 1 (HPRT1);

⁵GenBank accession number. qRT-PCR: Quantified by real-time reverse transcription-polymerase chain reaction.