Role of cytokine receptor-like factor 1 in hepatic stellate cells and fibrosis

Figure 4 Cytokine receptor-like factor 1 decreases expression of type III collagen by hepatic stellate cells. A. Expression of adenovirus delivered cytokine receptor-like factor 1 (CRLF1) in the cellular medium. HEK293 cells were transduced with control or CRLF1 expressing adenovirus. One day after transduction, CRLF1 protein was measured in the cellular medium by Western blotting; B: Stimulation of hepatic stellate cells (HSCs) with CRLF1. HSCs at day 2 after isolation were transduced with control or CRLF1 adenovirus. At day 7, expression of α-smooth muscle actin (α-SMA), collagen α1 (I), collagen α2 (I), collagen α1 (III) and fibronectin (FIB) mRNAs was analyzed by reverse transcription-polymerase chain reaction. CON: Control; COL1A1: Collagen, type I, α1; COL1A2: Collagen, type I, α2; COL3A1: Collagen, type III, α1.